HI,
CellMasks has worked for us with Hek 293 and hepg2 with rapid post-stain
imaging. Calcein am was also used to stain cell volumes, although it leaks
it is also preferable to image in the first hour post-staining.
M

-----Original Message-----
From: Cameron Nowell [mailto:[log in to unmask]] 
Sent: August 6, 2009 7:38 PM
To: [log in to unmask]
Subject: Whole Cell Dye

Hi List

Can anyone recommend a dye (or antibody) that will stain the whole cell
evenly to the plasma membrane? I need a dye that will give fairly even
staining across the whole cell to make segmentation easier. 

I have tried CellMask but is it a bit variable in its staining pattern,
there are blobs in the cytoplasm and nucleus may or may not stain up.
Something that stains just the plasma membrane would also be suitible. I
have used the invitrogen FX dyes before (not for this experiemnt) and
have found them a bit hit and miss too. I have also tried WGA but it
doesn't give an even stain around the mebrane which makes segmentation
quite dificult.

Any suggestions would be greatly appreciated.


Thanks

Cam


Cameron J. Nowell
Microscopy Manager 
Central Resource for Advanced Microscopy 
Ludwig Institute for Cancer Research 
PO Box 2008 
Royal Melbourne Hospital 
Victoria, 3050 
AUSTRALIA 
Office: +61 3 9341 3155 
Mobile: +61422882700 
Fax: +61 3 9341 3104 
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