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September 2013

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Subject:
Typical gain (in e-/ADU) for an Andor EMCCD camera
From:
Christophe Leterrier <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 3 Sep 2013 15:03:34 +0200
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Hi,

I'd like to evaluate the localization precision for single molecule
experiments. I need to convert the acquired signal into a number of
photons, using knowledge of the camera gain. Anyone would know what is the
typical gain (I mean the e-/ADU gain before taking into account the EM
gain) for an Andor Ixon 897 camera, using the 10 MHz readout and the gain
set to 1?

I'll try to ask Andor for my specific camera but that would be great to
have a number to try and see if my calculation is not too far off.

Thanks,

Christophe

--
Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France

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