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Dear all,

One thing we've tried is Cygel from (forgotten).  Melts when cold,
solidifies when warm.  Only drawback for us, apart from the huge cost, is
that it's only sold made up in PBS, rather than the pure compound.  May be
worth considering as a more permanent mountant, if the coverslip is sealed
after mounting.

cheers,
Rosmeayr

Rosemary White                    [log in to unmask]
CSIRO Plant Industry            ph.     61 (0)2-6246 5475
GPO Box 1600                       fax.     61 (0)2-6246 5334
Canberra, ACT 2601 
Australia



On 24/10/07 12:47 AM, "Judy Trogadis" <[log in to unmask]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Is sample shrinkage a general problem with mounting media which harden? I am
> not a fan of the PBS+glycerol+antifade combo because it's so messy - difficult
> to remove immersion oil, broken bits of nailpolish all over the stage and the
> lenses . . . . .
> 
> Judy
> 
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 7Queen
> 30 Bond St.
> Toronto, ON M5B 1W8, Canada
> ph:  416-864-6060  x6337
> pager: 416-685-9219
> fax: 416-864-6043
> [log in to unmask]
> 
> 
>>>> Sarah Aubut <[log in to unmask]> 10/23/07 8:56 AM >>>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> I recently attended a short course on Basic confocal at the Microscopy
> meeting in Fort Lauderdale, and the instructors advised against using
> Prolong and all other mountants that contain polyvinyl alcohol as this
> causes shrinkage up to 50% in your sample.
> What they suggested was something that matched the RI of the sample. One
> specific was PBS + glycerol (30%) with 0.25% p-phenylenediamine. DABCO was
> also mentioned as an alternative.
> I don't know if this information helps at all, but I thought I should
> contribute the little that I know!
> 
> 
> Sarah Aubut
> Lab Technician, Microscopy Suite
> Canadian Forestry Service
> Sault Ste Marie, ON
> Canada
> 
> [log in to unmask]