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Hi all -

I'm looking for some good review articles or books discussing fixing, 
clearing, and mounting methods for confocal microscopy.  A lot of our 
users are doing pretty simple things like mounting fixed and stained 
tissue in vectashield.  This sometimes gives pretty good images, but 
other users who use more elaborate techniques often get far better 
results.  I've learned some pretty useful methods this way, and also 
from reading the Handbook, but I figure (hope?) that there must be a 
good reference on sample preparation for immunofluorescence in confocal 
microscopy.  I'm primarily interested in preparation of tissue sections 
and whole mounts (thick samples) but if there are good reviews on 
preparation of thin samples I won't turn them down. 

If you know of any good reviews, books, or other references on this 
subject, please let me know.

Thanks,
Kurt

-- 
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu
phone 415.514.9709
fax   415.514.4300