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Dear Friends,



Reminder - recently two organizations (HSC & BINA) announced to support the upcoming FRET, FLIM and metabolic imaging workshop to provide partial tuition fee support. Please visit the web site to learn more about the support. https://kcci.virginia.edu/workshop-2024



Registration is open for the 21st Annual Workshop (in person) on FRET, FLIM, and Metabolic Imaging Microscopy, March 4-8, 2024.



https://kcci.virginia.edu/workshop-2024



The W.M. Keck Center for Cellular Imaging, a University of Virginia Imaging Center is sponsoring an advanced hands-on training course on fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging (FLIM), and Metabolic Imaging (NADH/FAD/Tryptophan). In metabolic imaging, the focus will be on the fluorescence lifetime redox ratio (FLIRR), a novel metric to investigate both, mitochondrial OXPHOS and cytosolic glycolysis and quantitative data analysis.



Also, limited amount of financial assistance is available with the support from BINA (Bioimaging North America).



Hands-on instruction and training on confocal, Spectral, lifetime (FLIM) FRET, and Metabolic imaging (FLIRR) Microscopy systems.



Discussion on best FLIM, FRET, and metabolic imaging (FLIRR) and data analysis solutions.



Personal attention for a maximum of 20 participants.



Internationally recognized faculty.



Individual problem solving.



*        For attendees to experience an advanced practical course on Förster (fluorescence) resonance energy transfer FRET) and fluorescence lifetime imaging (FLIM) microscopy to image the protein-protein interactions.



* In metabolic imaging, the focus will be on the fluorescence lifetime redox ratio (FLIRR), a novel metric to investigate both, mitochondrial OXPHOS and cytosolic glycolysis, accompanied by a quantitative analysis package which attendees will be trained on at the workshop.



*        Brief introductions to the principles of fluorescence, microscopy,  fluorophores, FRET, FLIM, and NADH/FAD/Tryptophan will be made. Emphasis will be on practical aspects, hands-on individual instruction at the instruments followed by data analysis and interpretation.



*        Problem-solving discussions will address questions of fluorophore choices, the most suitable systems to achieve specific research objectives, qualitative vs. quantitative analysis and much more. Introduction for a unique image processing and analysis software for FRET (PFRET), FLIM, and FLIRR will be given for training.



*        Live-cell specimens provided; participant's own specimens are welcome.



Please contact me off the list if you have any questions or clarifications.



Best,

Ammasi


Dr. Ammasi Periasamy
(he/him/his)
Director and founder of WM Keck Center for Cellular Imaging,
Prof. of Biology & Biomed. Eng., University of Virginia,
Physical & Life Sciences Building (PLSB 005)
90 Geldard Dr., Charlottesville, VA 22904, USA.

http://www.kcci.virginia.edu/people/profile/ap3t
Phone: (434) 243-7602 or 982-4869
E-mail: [log in to unmask]<mailto:[log in to unmask]>

21st Annual FLIM, FRET and FLIRR (Metabolic Imaging) Microscopy Workshop-March 4-8, 2024.  https://kcci.virginia.edu/workshop-2024