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August 2012

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Subject:
two photon fluorophores and mounting media
From:
"Ingaramo, Maria (NIH/NIBIB) [F]" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 15 Aug 2012 18:01:48 -0400
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Hello everyone!

I have two questions.

I am trying to image microtubules in fixed cells using tunable two photon excitation. I am interested in optimizing signal, and was wondering if anyone had suggestions regarding really good two photon excitable dyes for immuno-staining them. Alternatively, I could transfect with a fluorescent protein fusion. 

Also, I have tried imaging Alexa-488 and EGFP labeled tubulin in fluoromount G, and I am not happy with the results obtained in this media. Does anyone have a favorite mounting media that yields good images upon two photon excitation?

Thank you!

-maria

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