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This is purely a sectioning issue. Make thin sections of a bowl of spaghetti and you think you have a bowl of rice.
John
--
John J. Lemasters, MD, PhD
Professor and GlaxoSmithKline Distinguished Endowed Chair
Director, Center for Cell Death, Injury & Regeneration
Departments of Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular Biology
Medical University of South Carolina
DD504 Drug Discovery Building
70 President Street, MSC 140
Charleston, SC 29425
Office: 843-876-2360
Lab: 843-876-2354
Fax: 843-876-2353
Email: [log in to unmask]
http://academicdepartments.musc.edu/ccdir
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Engstrom, Lars
Sent: Monday, September 26, 2011 3:45 PM
To: [log in to unmask]
Subject: Re: Mitochondria: Fluorescence Microscopy vs. SEM
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That was the answer I was looking for!
Thanks.
-Lars
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of James Denegre
Sent: Monday, September 26, 2011 12:42 PM
To: [log in to unmask]
Subject: Re: Mitochondria: Fluorescence Microscopy vs. SEM
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Lars,
The textbook images are an inaccurate representation of the 3D nature of mitochondria. Mitochondria usually exist in reticulated networks, not as individual "beans" as they are often illustrated in text books.
The "bean" image is a misrepresentation of SEM data; the SEM data are from cross-sections of arms of the reticulated network, and do not represent the entire structure. If an extensive serial reconstruction were performed on SEM data a reticulated network would be appear.
Reticulated networks of mitochondria are well-illustrated in:
Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy.Plecitá-Hlavatá L, Lessard M, Santorová J, Bewersdorf J, Jezek P. Biochim Biophys Acta. 2008 Jul-Aug;1777(7-8):834-46.
4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet beta-cells, an experimental model of type-2 diabetes.
Dlasková A, Spacek T, Santorová J, Plecitá-Hlavatá L, Berková Z, Saudek F, Lessard M, Bewersdorf J, Jezek P, Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):1327-41.
Mitochondria, redox signaling and axis specification in metazoan embryos Coffman J, Denegre J, Developmental Biology 308 (2007) 266 280.
Regards,
Jim
James Denegre, Ph.D.
Senior Manager
Imaging Sciences
The Jackson Laboratory
Bar Harbor, ME 04609
207.288.6648
On 9/26/11 2:48 PM, "Engstrom, Lars" <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Maybe it is merely a magnification/resolution or optical slice
> difference. The cartoon kidney bean-like textbook figures closely
> approximate the SEM images where as fluorescent images surprised me at
> their length and "wormy-ness" (I know very scientific).
>
> I gave a presentation about DNA, Cells and Art to my daughter's 5th
> grade class. I was showing some images I have taken of mitochondria
> and couldn't definitively explain why they looked so different from
> the textbook images.
>
> -Lars
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Lemasters, John J.
> Sent: Monday, September 26, 2011 11:25 AM
> To: [log in to unmask]
> Subject: Re: Mitochondria: Fluorescence Microscopy vs. SEM
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Different how? I don't see a difference.
>
> --
> John J. Lemasters, MD, PhD
> Professor and GlaxoSmithKline Distinguished Endowed Chair Director,
> Center for Cell Death, Injury & Regeneration Departments of
> Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular
> Biology Medical University of South Carolina
> DD504 Drug Discovery Building
> 70 President Street, MSC 140
> Charleston, SC 29425
>
> Office: 843-876-2360
> Lab: 843-876-2354
> Fax: 843-876-2353
> Email: [log in to unmask]
> http://academicdepartments.musc.edu/ccdir
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]
> On Behalf Of Engstrom, Lars
> Sent: Monday, September 26, 2011 2:16 PM
> To: [log in to unmask]
> Subject: Mitochondria: Fluorescence Microscopy vs. SEM
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello -
>
> Since the first time I fluorescently stained mitochondria, I have
> always wondered why the structure of mitochondria look so different
> from the classical SEM images. Can someone please explain?
>
>
>
> Thank you for your time.
>
> -Lars
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