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** Vendor reply **

Dear Bruno,

Thank you Bruno from raising this question, and thank you Priyam for 
your immediate reply.

I am not sure how you obtained this price, but for adding the PSF 
distiller to Huygens Essential or Professional, we ask 2340 Euro.

For this price, you not only receive the option to distill a PSF from 
bead images. You get a lot more functionality for that:

- Multiple bead images can be loaded an, and each image may contain one 
or even multiple beads that are automatically detected

- A complete quality is performed on the loaded bead data to make sure 
than beads that are not suited are not taken into account for 
determining a PSF. For example, beads which blurring cones overlap or 
are not imaged, which are not passing a size check, and which are too 
close to the image edges receive a visible color-mark and are 
disregarded by the PSF Distiller Wizard.

Also, we perform a check on sampling, correct xyz dimensions, and 
illumination instability.

- Beads are averaged, and a PSF is distilled which is centered in the 
image volume and normalized.

This normalization step makes that the total SUM of the image is exactly 
1, which is crucial when performing a deconvolution with it, because it 
ensures that it basically does not add or remove signal from the image 
that is deconvolved

Images from which PSFs are distilled may contain up to 32 channels, and 
32 detectors images, and we support PSFs from a variety of microscope 
systems (STED, Spinning disk, widefield, confocal etcetera)

Support for 32 detectors allows you to distill a PSF form Array detector 
data, like the Zeiss Airyscan and SPAD data.

- Chromatic shifts are reported which can be used to chromatically 
corrects shifts between channels.

- FWHM values of the distilled PSF are calculated on-the-fly in xyz.

- A variety of curve-fitting models are available to determine the FWHM 
values.

- FWHM values can directly be compared with the theoretical PSF (per
channel and detector).

- A full Report is easily exportable to .csv or .txt file for monitoring 
microscope

- And finally, the PSF can be saved with the correct metadata (Array 
Detector, STED etc).

I like to make use of this thread to announce that a new Huygens Image 
Quality Control option will be released soon, which will be linked to 
all our restoration options, including the PSF Distiller, and produce 
even more PSF quality parameters.

Feel free to contact me or one of my colleagues directly if you have any 
further questions.

With kind regards,

Vincent


Vincent Schoonderwoert, PhD
Director of Marketing and Science

Scientific Volume Imaging - Makers of the Huygens Software

Website: svi.nl


On 07-02-2024 16:59, saubameb wrote:
> *****
> To join or leave the confocal microscopy listserv or to change your 
> email address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Priyam,
> thank you for your answer. I know about this option in Huygens. 
> However, to do so, you need to buy the "PSF distiller" option which is 
> very expensive (7000 €!!!). So I'm wondering whether it is possible to 
> work around....
> bruno
>
> Le 07/02/2024 à 16:22, Priyam Banerjee a écrit :
>> *****
>> To join or leave the confocal microscopy listserv or to change your 
>> email address, go to:
>> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
>> Post images on http://www.imgur.com and include the link in your 
>> posting.
>> *****
>>
>> Huygens deconvolution wizard has an option to move forward with
>> theoretical PSF or browse for measured PSF files.
>>
>> On Wed, Feb 7, 2024 at 4:37 AM saubameb 
>> <[log in to unmask]>
>> wrote:
>>
>>> *****
>>> To join or leave the confocal microscopy listserv or to change your 
>>> email
>>> address, go to:
>>> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
>>> Post images on http://www.imgur.com and include the link in your 
>>> posting.
>>> *****
>>>
>>> Dear all,
>>>
>>> is there a way to feed Huygens deconvolution software with a PSF 
>>> without
>>> using the PSF distiller option?
>>>
>>> In other words how should I process a z-stack of fluorescent microbeads
>>> (100 nm, tetraspeck) to obtain a PSF which I then could use for
>>> deconvolution in Huygens?
>>>
>>> All the best
>>>
>>> bruno
>>> -- 
>>> *Bruno SAUBAMEA *
>>> Maître de Conférences
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>>> picmo.u-paris.fr <https://picmo.u-paris.fr/>
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