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I would certainly recommend that you take a look at ImageJ, a free
open source package written in Java, with a large community of users
who share experience quite willilngly.

http://rsb.info.nih.gov/ij/

Joel

Date sent:              Fri, 18 Feb 2005 18:07:44 -0500
Send reply to:          Confocal Microscopy List <[log in to unmask]>
From:                   "Spurmanis, Aleks" <[log in to unmask]>
Subject:                cell counting
To:                     [log in to unmask]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi everyone,
>
> I'm looking for imaging software that can allow me to rapidly quantify
> the relative numbers of astrocytes (immuno-stained with AF488 tagged
> Abs to GFAP) and neurons (immuno-stained with AF546 tagged Abs to
> MAP2) in a primary culture of embryonic mouse cortical tissue.  I was
> planning to image the cells using WF epifluorescence and count the
> number of cells in both fluorescence channels in 3-4 visual fields
> using a low power objective (e.g., 10x).  I believe that I could
> perform this analysis quite easily with Image Pro Plus, but was
> wondering if anyone could recommend a less expensive alternative
> (MACE?) that will still get the job done?  In addition, I welcome any
> comments or suggestions to improve my methodology (e.g., switching to
> a multi-parameter fluorescent microplate assay).
>
> Thanks,
>
> Aleks Spurmanis
> Confocal Core Facility Manager
> Institute for Nutrisciences and Health
> 93 Mount Edward Road Suite M3-11
> Charlottetown PE
> C1A 5T1
> tel:  (902)- 566-7457
> fax: (902) - 569-4289
> e-mail:  [log in to unmask]
> <mailto:[log in to unmask]>
>
>

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [log in to unmask]