We have found that the Alexa Fluor 488 dye (formerly Alexa 488) shows the greatest
photostability when used with the Prolong antifade reagent.  This pdf file shows more
information:

http://www.probes.com/media/pis/td006.pdf

Here is the information on Prolong antifade:

http://www.probes.com/servlets/product?region=Select+Region&item=7481
--
Jill Hendrickson, Ph.D.
Molecular Biology Product Manager
Molecular Probes, Inc.
Phone:  541-465-8356
Fax:    541-465-4593



"Richard E. Edelmann" wrote:

>         We seem to be having a problem with Alexa 488 fading out *Really* fast (i.e.
> under 20 seconds).
>
> (1) Alexa 488 Goat Anti-rabbit (primary anti-body against a nuclear protein in
> Arabidopsis). Preparation at pH 8.0.  DABCO antifade.  {Counter staining with
> Propidium Iodide and it is also fading fast.  Bad DABCO?}
>
> (2) In comparison have done experiments with Alexa 488 Goat anti-Mouse (mRNA
> labeling), again DABCO antifade.  The Alexa signal was stronger and signal lasted
> much longer (minutes).
>
>         Any suggestions?
>
>         Thanks
>
> Richard E. Edelmann, Ph.D.
> Electron Microscopy Facility Supervisor
> 352 Pearson Hall
> Miami University, Oxford, OH 45056
> Ph: 513.529.5712        Fax: 513.529.4243
> E-mail: [log in to unmask]
>
> "RAM disk is NOT an installation procedure."