CONFOCALMICROSCOPY Archives

July 2008

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Subject:
Re: Alexa 546 vs 555
From:
Kurt Thorn <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 28 Jul 2008 13:54:59 -0700
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Alexa 555 has a pretty low quantum yield.  I generally prefer 546, 
though I've never used either with a 543 laser.

Kurt

MODEL, MICHAEL wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear List
>
>  
>
> Which Alexa dye do people prefer for use with a 543 laser, Alexa 546 
> or 555? Thanks!
>
>  
>
> Mike Model
>
> Kent State University
>
>  
>
>  
>


-- 
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu
phone 415.514.9709
fax   415.514.4300

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