Hi, Julie,

Wrong concept: these were actual pieces of metal, about 1/16th inch thick,
not foil. I know there was another step to the sample prep but was not in
attendance for that part of the lab.  I don't know how or even if the metal
was attached to the slide. I don't think so, since the spacers were
reusable.  I do remember their doing something, however, so that the fluid
stayed "in the groove".  Again, Nina Allen is your best source.  If you
have trouble finding her, let me know and I'll track her down.

Best regards,
Barbara Foster


At 04:04 PM 9/23/98 -0400, Julie R. Hens wrote:
>Dr. Foster,
>Did you only one layer of foil? or multiple layers? and Did you glue them
>to a slide?
>
>Thanks
>Julie
>
>On Wed, 23 Sep 1998, Barbara Foster wrote:
>>
>> Nina Allen (Wade-Forest, now at NCU or NC State) made some great spacers
>> from aluminum. I first saw them some years ago at her course at Woods
>> Hole.  They were cut about 1" x 2", with a channel in the middle for your
>> sample (essentially a squarish "U") and, as I remember, were used with
>> the large, rectangular coverslips
>>
>> Barbara Foster
>>
>>
>>
>> At 11:29 AM 9/23/98 -0500, Carol Bayles wrote:
>>
>> >Pat,
>>
>> >I use a very simple method.  I break up coverslips and use them as
>> spacers.
>>
>> >They are very uniform and come in varying thicknesses.  You can always
>>
>> >double them if necessary.  Just be careful not to cut yourself!
>>
>> >
>>
>> >Carol
>>
>> >
>>
>> >>I am trying to stain whole heart valve leaflets, with some
>>
>> >>success. However, mounting the end result in order to view, is
>>
>> >>proving difficult. Each leaflet is of variable thickness i.e. it
>>
>> >>has an undulating surface. Does anyone have any suggestions? I
>>
>> >>have tried making supports on a glass slide with mounting fluid or
>>
>> >>nail varnish, but it is difficult to end up with a uniform depth.
>>
>> >>Thanks in advance,
>>
>> >>Pat Taylor.
>>
>> >
>>
>> >
>>
>> >Carol Bayles
>> 607-254-4860
>>
>> >Microscopy and Imaging Facility         607-254-4847  fax
>>
>> >BioResource Center                              [log in to unmask]
>>
>> >160 Biotech Bldg
>>
>> >Cornell University
>>
>> >Ithaca NY 14850
>>
>> >
>>
>> >
>>
>
>