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Dear List--

When deconvolving datasets, it's sometimes necessary to know the size of
the pinhole as back-projected to the plane of the specimen.  This can be
done easily if you know the total magnification of the system, from the
objective up to the plane of the iris.  However, actually measuring that
value is tricky.

Ignoring the objective itself, does anyone know what the mag of the
system is for a BioRad 1024?  There's an 8x photo-ocular that
contributes to this value, but the system adds more mag by projecting
the image of the confocal spot for a long distance--rather like a film
projector, which makes a bigger image if the screen is further away.

My understanding is that the value for the MRC 600 was 53 (exclusive of
any other contributions by transfer lenses in the fluorescence
illuminator, for instance).  Does anyone know what this value "should"
be for an MRC 1024?

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                       office:  (612) 626 0145
Assoc Prof, Dept Neuroscience                     lab:  (612) 624 2991
University of Minnesota                 Preferred FAX:  (612) 624 8118
6-145 Jackson Hall, 321 Church St. SE        Dept FAX:  (612) 626 5009
Minneapolis, MN  55455               e-mail:  [log in to unmask]