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Dear all,

An investigator wants to know the best technique to fix tissue for the
preservation of DsRed fluorescence.

They have a probe/plasmid coupled to DsRed and they want to study the
distribution (I don't know all of the particulars) of cells labeled
(stablely transfected) with this probe in various mouse organs.  Cells
are injected into the mice i.v.

After certain periods of time, organs (liver, spleen, etc.) are removed,
fixed in 4% paraformaldehyde overnight, then 2 to 3 hrs in 30% sucrose,
and finally embedded in OCT for sectioning.

Does anyone have any suggestions about how this method can be improved
with regard to fluorescence preservation?

Thanks for any help.

Ray Hester
Univ. of South Alabama
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