Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi there,
The freeware ImageJ has an image correlation plugin (a new version from
ImageJ's authors is in the pipeline that will work on stacks). It
generates scatterplots and a three column data table (channel 1
intensity, channel 2 intensity and pixel frequency). This can be put in
to excel to calculate coefficients.
I've finished modifying my own version of the plugin which works on
stacks (it runs slower than the author's version though) and also
generates Pearson's coefficents, Overlap coefficients and colocalisation
coefficients based on Manders 1993 J. Microscopy paper (a more
accessible summary of this paper can be found as a mediacybernetics tech
note
http://www.weiss-imaging.de/Web_PDF/Applikationen/colocfluorprobes.pdf).
What other measures of colocalisation are people using? I'd appreciate
any references to other coefficients.
If anybody would like the plugin please email me. I'd really appreciate
it if anybody using ImagePro Plus and ImageJ would contact me to verify
that the numbers my plugin generates match those generated by ImagePro
Plus. I've run one stack through both programs and the numbers match,
but I'd like to be sure.
Regards
Tony
Glen MacDonald wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Joe,
> The website had to be taken down because NIH was dragging it feet with
> the technology transfer paperwork. I'm surprised that has,n't been
> completed as Peter Bergland ([log in to unmask]) was optimistic that would
> have been completed by this fall. I don't have his other website
> address at hand, but just email Peter.
>
> Many other applications have colocalization, but this is about as easy
> to use and cost effective as it gets. The downside is that it is a 2D
> program that will work with stacks. Some of the more modules in more
> expensive applications will perform 3D co-localization on stacks.
>
> Regards,
> Glen
> On Dec 10, 2003, at 8:26 AM, Goodhouse, Joseph wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Who posted this message a while back. The url takes you no where .
>> Could
>> you please provide the correct url?
>>
>> No web site is configured at this address.
>>
>> CoolLocalizer now available!
>> CoolLocalizer is a professional image analysis software
>> for microscopy users. Example of features:
>>
>> Very easy to use and learn, designed by
>> microscopy users.
>> Illustrative fluorogram analysis of RGB channels.
>> Quantitative co-localization analysis,
>> mathematically solid algorithms.
>> Intuitive selection tools including novel Quadrant
>> analysis.
>> Z-stacks, times series, and sequentially aquired
>> images.
>> File format conversion including Biorad .PIC,
>> Leica TIFF etc.
>> Montage layout for presentations and
>> publications.
>>
>> New Version Available - CoolLocalizer
>> 1.0.5
>> More info and demo at
>>
>> www.coollocalizer.com
>>
>> Joe Goodhouse
>> Confocal / EM Core Laboratory
>> Department of Molecular Biology
>> Princeton University
>> 609-258-5432
>>
>> Visit us at
>> http://www.molbio.princeton.edu/facility/confocal/index.html
>>
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923 USA
> (206) 616-4156
> [log in to unmask]
>
> ************************************************************************
> ******
> The box said "Requires Windows 95 or better", so I bought a Macintosh.
> ************************************************************************
> ******
--
Tony Collins Ph.D.
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto M5T 2S8
tel. (416) 603 5367
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