Hi Christophe,
> So I ended up mounting my cells in PBS( +n-propyl-galate as
> an anti-fade), so that the RI is 1.33. I struggled to find a
> good sealant that allowed me to keep my slides (PBS is not
> viscous like PBS/glycerol and evaporates very rapidly). I
> tested VALAP, DPX but none were impermeant enough to avoid
> evaporation after a few days... So if you have a proposition
> I'd be glad to hear it.
Try to store your slides in a humidity chamber on a support located above
the mounting buffer. The mounting buffer (PBS in your case) will provide the
appropriate humidity / vapor pressure to equilibrate the atmosphere /
exchange with your sample.
Please check in advance if your sealant will solubilize in this humid
atmosphere...
Hope this helps
Guenter
>
> Christophe Leterrier
>
>
> On Wed, Mar 25, 2009 at 17:37, Glen MacDonald
> <[log in to unmask]> wrote:
>
>
> I've read that Fluoromount G, a hardening mountant from
> Southern Biotech, has been measured to an RI of about 1.338.
>
> Regards,
> Glen
> On Mar 25, 2009, at 6:40 AM, Sylvie Le Guyader wrote:
>
>
>
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>
> Hi everyone
>
>
>
> I am trying to find some mounting medium with a
> refractive index close to the one of water (1.33) in order to
> use our ZDC (Olympus zero drift control, IR-based hardware
> autofocus) with fixed samples.
>
>
>
> Currently we use Dako S3023 and the focus is
> regularly lost without apparent reason. Andor, who sold us
> the system, claims that it could be because the IR of the
> mounting medium doesn’t contrast enough with the one of
> glass. I asked Dako for the RI of S3023 and they repeatedly
> stated 1.47.1-5 which is pretty vague but is definitely not
> 1.33. However I have found here on the confocal server that
> the RI of Dako S3023 had been measured and would be 1.372.
>
>
>
> Does anyone know what the Dako RI is and where
> I could find a mounting medium that hardens and has an RI
> close to 1.33? does anyone have any experience with imaging
> fixed and mounted cells with a hardware autofocus?
>
>
>
> Thanks for your help!!
>
>
>
>
>
> Med vänlig hälsning / Best regards
>
>
>
> Sylvie
>
>
>
> @@@@@@@@@@@@@@@@@@@@@@@@
>
> Sylvie Le Guyader
>
> Dept of Biosciences and Nutrition
>
> Karolinska Institutet
>
> Novum
>
> 14157 Huddinge
>
> Sweden
>
> +46 (0)8 608 9240
>
>
>
>
>
>
>
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