Hi Sylvie

We're also offering our BOX incubators in black 
or any combination of transparent and black (commercial interest, of course).

Best regards,

Beat

At 10:22 14-05-2009, you wrote:
>I think Olympus has now implemented the tuning of the laser and the
>compensation within their software.
>
>Cameron, where did you get these black incubators? I am interested in
>getting one done as well.
>
>Med vänlig hälsning / Best regards
>
>Sylvie
>
>@@@@@@@@@@@@@@@@@@@@@@@@
>Sylvie Le Guyader
>Dept of Biosciences and Nutrition
>Karolinska Institutet
>Novum
>14157 Huddinge
>Sweden
>+46 (0)8 608 9240
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[log in to unmask]] On Behalf Of Cameron
> > Nowell
> > Sent: 14 May 2009 00:28
> > To: [log in to unmask]
> > Subject: Re: Recommendations for commercial multi-photon system purchase
> >
> > Hi Lisa,
> >
> > Thought i woudl chip in with my experience as well. We have 2 Olympus MPE
> > Systems, one inverted and one upright that share a common Mai Tai Laser
>(the
> > laser can only be sent to one scope at a time). The upright is used
>routinely for
> > intravital imaging and has performed flawlessly. We have imaged zebra fish
>and
> > mice (both live and dead) on it and acheived some spectacular data sets.
> >
> > The current configuration has on large 0.95NA water imersion 20x objective
>on it,
> > but it is easy enough to slip another turret of objectives onto it if
>required. Not sure
> > if it is true for other system but the olympus system is tuned to work
>with the 20x
> > objective in multiphoton mode, so putting other objectives on may not
>result in
> > optimal imaging. For single photon excitation it doesn't matter.
> >
> > The software is stable and easy enough to use. Users are usually up and
>going
> > independently after a coupel of hours of training and practice.
> >
> > The only issue i have with it is that you can not communicate directly
>with the laser
> > via the Olympus software. You can control the laser power via an ND filter
>system
> > but other functions (such as wave length tuning) are carried out using a
>seperate
> > software package installed on a laptop plugged into the laser.
> >
> > One last thing worth mentioning is that both microscoes are fitted with
>black acrylic
> > incubators (thanks to Steve Cody for this). They work so well at blocking
>out light
> > that you can perform MP imaging with the room lights on!
> >
> > Also look into what the service provided by the companies are in your
>area. Here in
> > Melbourne Olympus are probaly one of the best for response and quality of
>service.
> > There is no point getting the best microsocpe out there if the peopel in
>your area
> > are not that good at keeping it going.
> >
> >
> > Cheers
> >
> >
> > Cam
> >
> >
> >
> > Cameron J. Nowell
> > Microscpy Manager
> > Central Resource for Advanced Microscopy
> > Ludwig Insttue for Cancer Research
> > PO Box 2008
> > Royal Melbourne Hospital
> > Victoria, 3050
> > AUSTRALIA
> >
> > Office: +61 3 9341 3155
> > Mobile: +61422882700
> > Fax: +61 3 9341 3104
> >
> > http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
> >
> >
> > ________________________________
> >
> > From: Confocal Microscopy List on behalf of Cameron, Lisa
> > Sent: Thu 14/05/2009 12:04 AM
> > To: [log in to unmask]
> > Subject: Recommendations for commercial multi-photon system purchase
> >
> >
> >
> > I have been investigating commercial multi-photon systems for awhile in
>order to
> > purchase a system for my Institute's core microscopy facility. Our
>interest is to have
> > the capability to do intravital imaging on an upright stand, but also be
>able to have
> > facility users be able to put slides on and use the visible scanner and
>detectors. I
> > realize this is a tall order for such versatility in one system, but since
>it is for a core
> > (which needs to bring in revenue), I'm looking for the most flexible
>system. Does
> > anyone have any suggestions about the most recent systems on the market?
>Or
> > could you point out factors you think are the most important for making
>the decision
> > on which company to go with? Please feel free to contact me off line.
> >
> > I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and
> > Prairie's system.
> >
> > (BTW - my own experience is with widefield and confocal live-cell imaging,
>so I
> > have not done 2-p myself, but have been learning about it for about a
>year)
> >
> > Thanks!
> > - Lisa
> >
> > ---------------------------------------
> > Lisa Cameron, Ph.D.
> > Director of Confocal and Light Microscopy
> > Dana Farber Cancer Institute
> > 44 Binney St.; JF 215
> > Boston, MA 02115
> > Office phone: 617-582-8824
> > Fax: 617-582-8750
> > [log in to unmask]
> >
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