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I must say that I continue to have problems with the Solent system. I
cannot get it to maintain the pH of standard unbuffered cell culture media
overnight. In this system, the 5% CO2 enters a humidification unit
(within the outer 37 degree chamber) and then is delivered to the working
head (the smaller sub-chamber that sits on the stage). It's possible that
the CO2 is leaking out from under the working head or through the bottom
of the stage. Currently, I am considering extreme measures to close the
system from below.
Evaporation can also be a problem with this system, especially during the
dry winter months.