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Dear Guenter,
Are you intending to perform your labeling by pre-embedding or
post-embedding methods?  If you can perform your fluorescent labeling
before embedding, many fluorophores are compatible with dehydration and
embedding in epoxy resins such as Spurr's and Epon (or alternatives).
The final RI will be in the range of 1.48-1.5.

Regards,
Glen

On Jun 7, 2005, at 1:56 AM, Guenter Giese wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear list members,
>
> which are the embedding media with highest refractive index (r.i. 1.48
> to
> 1.52) but still compatible with standard fluorochromes / fluorescent
> proteins?
>
> Ideally, the refractive index would match the r.i .of immersion oil
> (e.g.
> ZEISS Immersol 518 F: r.i. 1.518).
>
> Any reference welcome.
>
> Guenter
>
> ------------------------------------------
> Dr. Guenter Giese
> Light Microscopy Facility
> Dept. of Biomedical Optics, MPI fuer Medizinische Forschung
> Jahnstr. 29, D-69120 Heidelberg, Germany
> Phone (+49) 6221-486-360 (Fax: -325)
> e-mail: [log in to unmask]
> http://www.mpimf-heidelberg.mpg.de/~ggiese/lightmicro
>
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[log in to unmask]

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