CONFOCALMICROSCOPY Archives

June 2007

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Subject:
Re: stage top incubator
From:
Vickery Trinkaus-Randall <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 1 Jun 2007 17:00:07 -0400
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

cells will bleb and you will see ruffling much more in HEPES than media 
- looks beautiful but it is stress!

Jennifer Waters wrote:

> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>  
> Hi Jennifer,
>  
> We purchased and like heaters from 20/20 Technology 
> (http://www.20-20tech.com/).  Also, try Gibco CO2 Independent media 
> for imaging.  Lots of cells seem to like it.  Alternatively, 25mM 
> Hepes added
> to most medias will buffer without CO2.
>  
> Best, Jennifer
>  
> -- 
> Jennifer Waters, Ph.D.
> Director, Nikon Imaging Center at Harvard Medical School

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