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Dear all,

Thank you all for your help on and off list.

Zeiss has found the solution: they have updated the computer BIOS to a most recent version and the microscope has been working perfectly well since.

Sincerely,

Matthieu

From: VERMEREN Matthieu
Sent: 13 January 2021 15:45
To: [log in to unmask]
Subject: Camera issue

Dear all,

I'm reaching out as I have an issue with a camera:


Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu Flash4 camera linked to our Observer has started behaving strangely.  It is feeding images to the Zen software that do not correspond to what the microscope should see: if for example, we try to image DAPI, FITC and TRITC in a multichannel image, all channels look like DAPI.  Yet, the microscope changes filters appropriately.  It's as if the camera buffer is not emptying itself and re-feeding an old image.  Sometimes, these ghost images happen when there is no object on the stage.  Drivers are up-to date and computer is running Zen 2.6.



The Zeiss engineers are equally puzzled.



Has anyone experienced this issue before and if so, what was your solution?



Sincerely,



Matthieu

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