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Bill,

you should also look at the new Zeiss
LD LCI "Plan-Apochromat" 25x/0,8 Imm Korr DIC multiimmersion objective
which can be used with or without coverglass and has a working distance
of 570 microns when used with coverglass and without I think even more.
This can be used in the AxioImager without any problem as it has no
special length and is better corrected than the 20x. As long as you do
not have to use microinjection or patch-clamp it should be fine, at
least it works well in our hands.

Roland


Townend, Bill schrieb:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> We would like to try a Olympus objective( 20x/0.95 NA water dipping) with a 2mm working distance on our Zeiss AxioImager.Z1. The objective is 75mm long and seems to have a special nosepiece  with a 25m thread when it is used on an Olympus.  The Zeiss seems to have 27m thread. Does anyone know if there is an adapter for this objective? 
>  
> Does any have another suggestion for 2p work? We are looking for cell-cell interactions so a large area is useful.  Extra intensity is helpful.  We must be able to work at least 200 microns into tissue like lymph node. Zeiss is releasing a similar 20x with a 1.0NA so this is a possible expensive (15K) solution.
>  
> Thanks,
>  
> Bill Townend
> Cytometry Coordinator
> National Jewish Medical and Research Center
> 1400 Jackson St, K1014
> Denver, CO 80206
> 303 398-1330
> [log in to unmask]
>  
> 
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-- 
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Nitschke, Roland Dr.
Life Imaging Center
Institute of Biology
Albert-Ludwigs-University Freiburg
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