Dear Lazlo, chlorophyll  mostly fluoresces red. If you use a narrowish
     band pass filter for the GFP, you should be OK. Quite a few of our
     users have used GFP in plants. eg see Bio-Rad Application Note 17.

     Anna Smallcombe PhD (Bio-Rad Microscopy Division)


______________________________ Reply Separator _________________________________
Subject: GFP detection in green plants?
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet
Date:    02/12/98 11:07


Hi everybody,

We have a potential user who would like to use green fluorescent
protein (GFP) in his studies. The protein  would be expressed
in transgenic plants. The problem is that chlorophyll has a strong
fluorescent signal overlapping the 510 nm emission peak of the GFP
variant used.

I would appreciate receiving any hints or pointers to references
describing how to detect GFP fluorescence in green plant tissues
using LSM techniques.

Thanks!

Laszlo Menczel, Ph.D.
LSM Laboratory
Biological Research Center
Szeged, Hungary

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