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Hi,
Check these two possibilities.
1- The objective is not quite perpendicular to the X or y axis. You can see this if you use reticule.
2- Reboot the software to eliminate the bugs if you are lucky!
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Glen MacDonald
Sent: Tuesday, March 27, 2007 3:45 PM
To: [log in to unmask]
Subject: Image shift
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Hi,
I'm trying to diagnose an odd problem with an FV-1000 and IX-81 microscope, to get speed things in getting this serviced. When collecting a z-series, around 330 to 400 µm into the volume, the image shifts in the x-axis by several pixels to the left, sometimes 1 or 2 lines upwards. The shift may occur within 1 line, or up to 15 lines resembling a vibration, but trending to the left. in the subsequent portion of the stack, additional shifts will occur every 40 to 120 µm. If I capture the full volume in increments of less than 400 µm, the shift will still occur. I've used box sizes from
256 to 1024 at dwell times of 2 µs to 20 µs. At 2 µs with a 256x256 box, the shift doesn't occur within a frame, but seems to be between the frames. Also, the shift always occurs in the upper 1/3 of an image.
In line sequential acquisition, the shift appears in all channels.
With sequential frame acquisition, the shift appears within a frame of the first channel but the slice images for the other channels shift in their entirety.
The air table is up, although vibrations shouldn't be this regular.
Also, the starting point of the z-series may vary, relative to the coverslip.
Any suggestions?
Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA
(206) 616-4156
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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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