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Hi all,
I refer back to a thread from two years ago when 37C immersion oils were discussed.
We have been using the Cargille 37DF oil since then for live cell imaging.
However we have just been informed by the local supplier that Cargille has discontinued DF oils.
Does anyone know if there is a suitable replacement for use at 37C on a confocal.
Regards,
Adrian
______________________________________________
Dr Adrian Smith
Manager, Cytometry & Imaging Facilities
Centenary Institute
http://www.centenary.org.au
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.
Ph: 61-2-9565-6189 Fax: 61-2-9565-6101
On 23/03/2009, at 11:58 PM, [log in to unmask] wrote:
> Dear all,
>
> As someone who is so directly involved (being the first author of the publication mentioned below), at first I resisted reacting to this thread. Now I would like to mention that information about the 37DF immersion oil can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) and secondly, that those of you who might be interested in a pdf of our publication on this oil can write directly to me.
>
> Please note: NO commercial interest.
>
> Lauran
> --------------------------------------------------------------
> Lauran Oomen
> Manager Digital Microscopy Facility (H003)
> NKI-AVL
> Plesmanlaan 121
> PO Box 90203
> 1006 BE Amsterdam
> The Netherlands
>
> phone +31 205126080
> [log in to unmask]
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christophe Leterrier
> Sent: maandag 23 maart 2009 11:42
> To: [log in to unmask]
> Subject: Re: Immersion Oil - 37oC
>
> I would like to point out that Cargille has a new oil for fluorescence
> imaging at 37°C, called 37DF, that has very low auto fluorescence. It
> is not on their website, but I could get the product pdf sent to me by
> a Cargille person, so I can send it if someone is interested. I'm
> trying to get some here in France, but none of the french Cargille
> distributors has heard about it yet... It has been found to be quite
> good for fluorescence imaging of live cells at 37°C, according to this
> paper in J Microscopy :
>
> Immersion oil for high-resolution live-cell imaging at 37 degrees C:
> optical and physical characteristics.
> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K.
> J Microsc. 2008 Nov;232(2):353-61.
>
>
> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[log in to unmask]> wrote:
>>
>> Hi all,
>>
>>
>>
>> Using immersion oil at 37oC.
>>
>>
>>
>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, 250 and 500 ml, unfortunately all filled with the same 518F oil. According to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little extra information is offered on the Zeiss website.
>>
>>
>>
>> The only info I can gleam from the web [other than the ubiquitous 518F safety data Sheet] is the following
>>
>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, n(e)=1.518 (23°C), halogen-free)
>>
>>
>>
>> So not much comment about 37oC use. PeCon's site [who supply most of Zeiss's decent incubator stuff] have no opinion on the matter either.
>>
>>
>>
>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: For application information, consult the processing instructions. Processing instructions or technical
>>
>> information sheet available on request.". I'll ask our knowledgeable local Zeiss Confocal rep for more details, as we are just installing a Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead confocal.
>>
>> Cargilee's site in comparison is passionate about all things immersion oil:
>> http://www.cargille.com/immeroil.shtml
>> http://www.cargille.com/immeroilselection.shtml
>> and their excellent, if elderly, 'primer'
>> http://www.cargille.com/immersionoilmicroscope.shtml
>>
>> From postings to this server back in 2006, and Cargille's site not mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 probably autofluoresces, so it's likely better for live cell phase-contrast [halogen lamp] transmission microscopy [but then often so are dry objectives]. I'll stick with standard 518F immersion oil at 37oC though, it's always imaged well enough on Zeiss microscopes at that temperature [and at least at 37oC it never became cloudy due to crystal precipitation]. Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick ruthlessly with the same brand on each microscope. A lot of our live cell work is/was done with low power [20x Phase] air objectives though.
>>
>> I do notice that immersion oil often ingresses into oil immersion objective internal optics with time, although strangely it rarely notices when imaging [like dirt on much of the internal optics, you must mostly focus through it]. One microscopy core manager even commented to me that he considers oil objectives 'consumables'. The cost of 'repair' [often the same price as a new objective] is so high we frequently have to live with it [I advise users that our say 40x objective image quality is suspect for this reason]. In truth we don't notice any drop in image quality when it must have happened, although you can see it 'smeared' on the optics when looking through the removed objective, using a magnifying glass, bright room light and a 'cleaned' objective top lens.
>>
>> Old listerver posting on Cargille 37 attached below.
>>
>> Regards
>>
>> Keith
>>
>> PS missed this link on the last post:
>>
>> Dissolving crystals in Zeiss 518F oil
>> http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf
>>
>> ---------------------------------------------------------------------------
>> Dr Keith J. Morris,
>> Molecular Cytogenetics and Microscopy Core,
>> Laboratory 00/069 and 00/070,
>> The Wellcome Trust Centre for Human Genetics,
>> Roosevelt Drive,
>> Oxford OX3 7BN,
>> United Kingdom.
>>
>> Telephone: +44 (0)1865 287568
>> Email: [log in to unmask]
>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/
>>
>>
>>
>> Subject:
>>
>> Re: Fluorescence from Type 37 Immersion Oil
>>
>> From:
>>
>> Vitaly Boyko <[log in to unmask]>
>>
>> Reply-To:
>>
>> Vitaly Boyko <[log in to unmask]>
>>
>> Date:
>>
>> Wed, 16 Aug 2006 10:23:20 -0400
>>
>> Content-Type:
>>
>> text/plain
>>
>>
>>
>> Search the CONFOCAL archive at
>>
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>
>>
>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) with
>>
>> the Nikon type A or Cargille type DF.
>>
>>
>>
>>
>>
>>
>>
>> Hi Jason,
>>
>>
>>
>> I wonder why you have tested the Cargille FF imm. oil, as the refractive
>>
>> index of FF oil is too far off for the standard glass (1.518) ?????!!!!!
>>
>>
>>
>> Actually, I haven't noticed the "very bad" autofluorescence of the Cargille
>>
>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The difference is
>>
>> less than 10%.
>>
>>
>>
>> The problem with the Nikon oil is its dispenser and/or viscosity - it is
>>
>> often annoying "fighting" with the air bubbles.
>>
>>
>>
>> What is about the Invitrogen/Mol.Probes mounting media autofluorescence?
>>
>>
>>
>> I am fed up with the irreproducibility of the background autofluorescence
>>
>> using the ProlongGold mounting media!!! That is a serious problem compared
>>
>> to the issue of the immersion oils.
>>
>>
>>
>> Cheers,
>>
>>
>>
>> Vitaly
>>
>>
>>
>> NCI-Frederick
>>
>>
>>
>>
>>
>> ----- Original Message -----
>>
>> From: "Kilgore, Jason" <[log in to unmask]>
>>
>> To: <[log in to unmask]>
>>
>> Sent: Tuesday, August 15, 2006 7:26 PM
>>
>> Subject: Re: Fluorescence from Type 37 Immersion Oil
>>
>>
>>
>>
>>
>>> Search the CONFOCAL archive at
>>
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>>
>>
>>> Back in 2000 I performed a comparison of four different immersion oils
>>
>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for
>>
>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter
>>
>>> sets) and general resolution at 60x on a Nikon E400. I didn't test
>>
>>> Cargille 37.
>>
>>>
>>
>>> Green autofluorescence tended to be worst for all four, but was least
>>
>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad)
>>
>>> Cargille DF. However, the resolution was unacceptable for Cargille FF.
>>
>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to
>>
>>> very low (acridine orange), with the same comparative trend applying.
>>
>>>
>>
>>> Thus, ever since we have been using Nikon Type A with no complaints for
>>
>>> most of our imaging.
>>
>>>
>>
>>> Feel free to contact me personally and I can send you the data.
>>
>>>
>>
>>> Jason
>>
>>>
>>
>>> Jason A. Kilgore
>>
>>> Cell Biology / Histology
>>
>>> Molecular Probes/Invitrogen
>>
>>> [log in to unmask]
>>
>>>
>>
>>>
>>
>>> -----Original Message-----
>>
>>> From: Confocal Microscopy List [mailto:[log in to unmask]] On
>>
>>> Behalf Of S. Brunet
>>
>>> Sent: Tuesday, August 15, 2006 11:19 AM
>>
>>> To: [log in to unmask]
>>
>>> Subject: Fluorescence from Type 37 Immersion Oil
>>
>>>
>>
>>> Search the CONFOCAL archive at
>>
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>>
>>
>>> Hello to all,
>>
>>>
>>
>>> We recently began using the Cargille 37 oil with the temperature chamber
>>
>>> on the
>>
>>> microscope. I found significant fluorescence from the oil when exposed
>>
>>> to the
>>
>>> HBO lamp (I could not see the eYFP because of the blue background).
>>
>>>
>>
>>> So I used the 20x air objective and I was able to obtain an image from
>>
>>> the
>>
>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample
>>
>>> (excitation at 488 or 458nm and pinhole at the usual confocal setting).
>>
>>>
>>
>>> Is this the norm?
>>
>>>
>>
>>> Regards,
>>
>>> Sophie
>>
>>> _______________________________________
>>
>>> Sophie M.K. Brunet, Ph.D.
>>
>>> Research Officer
>>
>>> Optical Spectroscopist, Laser Systems and Applications
>>
>>> [log in to unmask]
>>
>>> 306-966-1719 (office) 306-966-1702 (fax)
>>
>>> _______________________________________
>>
>>> Saskatchewan Structural Sciences Centre
>>
>>> University of Saskatchewan
>>
>>> Thorvaldson Bldg.
>>
>>> 110 Science Place
>>
>>> Saskatoon, SK S7N 5C9
>>
>>>
>>
>>
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