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Dear Marco,
The issues of phototoxicity and photobleaching usually force limiting  
light exposure as much as possible when working with live cells. Why  
would you choose a structured light imaging system that uses 3  
exposures per image over a deconvolution system that uses a single  
exposure?  the only advantage of structured light would be the speed  
at which one obtains the final image.  However, you might be rapidly  
obtaining sharp images of degrading samples.  There are many options  
for employing deconvolution, which seems more appropriate for live  
cells.   Fast computers and a little discipline in dataset size can  
greatly reduce the time required for the final result.

Regards,
Glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[log in to unmask]

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On Dec 1, 2005, at 12:15 PM, Marco Prado wrote:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi- 
> bin/wa?S1=confocal
> Hi
>
> I apologize as this is not a confocal question, but it is likely of  
> interest to confocal users.
>
> I was wondering whether somebody has compared a microscope equipped  
> with optigride with one having the Apotome from Zeiss. We plan to  
> use these systems with a Nikon TE-2000 inverted or an Axiovert 200   
> respectivelly, to decrease the work load of our confocal. Our work  
> is most on live cells, using GFP and other probes, but we do the  
> occasional brain slices with fluorescent probes. I am interested to  
> know about the quality of optical sectioning achieved by these two  
> systems; signal to noise;. Image processing or any problems people  
> may have found.
>
>  In addition the Nikon people are offering a new software for  
> acquisition NIS- elements, has anyone used or seen a demo for this  
> software?
>
>
>
> Thanks for the info
>
> Marco
>
>
>
> Dr. Marco Antonio M. Prado
>
> Associate Professor
>
> Department of Pharmacology, ICB
>
> UFMG, Av. Antonio Carlos 6627 Belo Horizonte, MG
>
> 31270-910, Brazil
>
> tel 55 31 34992718
>
> fax 55 31 34992695
>
>
>
>
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