CONFOCALMICROSCOPY Archives

January 2001

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Confocal Microscopy List <[log in to unmask]>
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Thu, 4 Jan 2001 20:37:38 -0600
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If no one has an answer, send me some and I will run it on a refractometer,
temperature controlled, etc.  This won't give you the delta t but I could
run a few different temperatures around what you plan to use.



At 03:33 PM 1/4/01 -0600, Tom Phillips wrote:
>I would like to expand Susan's question to ask if anyone knows the RI
>of Mowiol?  Even better, is there a reference or web site that lists
>the RI of common commercial and homemade mounting media.  Thanks, tom
>
>
>
>
>>Dear Confocalists,
>>
>>I would like to test the mounting medium called Permafluor. It is made by
>>Shannon Lipshaw, marketed by Immunotech in Europe, and available in the
>>U.S. from Beckman-Coulter, product #IM0752. B-C has not been able to get a
>>response, from the developer, to my questions . Does anyone know what the
>>refrative index of Permafluor is? How does it compare to Prolong Antifade?
>>Has anyone used the current formulation to mount thick (350 micron) tissue
>>samples?  We're looking at fixed, whole cricket ganglia.
>>Thank you,
>>
>>Susan Krall Krueger
>>Center for Computational Biology
>>Montana State University
>>Bozeman, MT   59717
>>406-994-7748
>--
>Thomas E. Phillips, Ph.D.
>Associate Professor of Biological Sciences
>Director, Molecular Cytology Core Facility
>
>3 Tucker Hall
>Division of Biological Sciences
>University of Missouri
>Columbia, MO 65211-7400
>(573)-882-4712 (voice)
>(573)-882-0123 (fax)

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: [log in to unmask],
Tel: 847-270-5888
Fax: 847-270-5897

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