TDE seems to unbind phalloidin, even if you fix again after staining. If anyone has found a
solution to phalloidin staining and TDE mounting it would be good to hear. Specimens are
mounted in TDE stepwise through a dilution series. TDE is diluted in PBS in concentrations
of 10, 25, 50 and 97% (the final concentration determines the R.I you want). I recommend
you check out the original paper that explains the simple procedure very clearly.
Paul