CONFOCALMICROSCOPY Archives

October 2008

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Paul Appleton <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 2 Oct 2008 11:43:46 -0500
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TDE seems to unbind phalloidin, even if you fix again after staining.  If anyone has found a 
solution to phalloidin staining and TDE mounting it would be good to hear.  Specimens are 
mounted in TDE stepwise through a dilution series.  TDE is diluted in PBS in concentrations 
of 10, 25, 50 and 97% (the final concentration determines the R.I you want).  I recommend 
you check out the original paper that explains the simple procedure very clearly.

Paul

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