Hi Manoj,
Ummmm.... i think you mean something like
Image (or the Fiji distribution of ImageJ)
http://pacific.mpi-cbg.de
or do you mean something else?
Can you describe what you mean in more detail?
Dan
On Jun 21, 2010, at 7:03 AM, CONFOCALMICROSCOPY automatic digest system wrote:
> Date: Sun, 20 Jun 2010 12:04:38 -0400
> From: manoj jaiswal <[log in to unmask]>
> Subject: Software to create raster plot and good combination of VSD and calcium dye
>
> --0016363b85da599f2504897858c4
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
>
> I was wondering if you could help me to find a decent tool to draw raster
> plot for the 2-photon images of neuronal network loaded with calcium dye.
> Which software packages are often used and hopefully free to download except
> matlab?
>
> Also which is best voltage sensitive dye one can use in combination with
> calcium dye (OGB-1/Fluo-4AM/Fura-2AM etc.) ?
>
> Thanks in advance,
> Manoj
Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany
+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
http://www.bioimagexd.net BioImageXD
http://pacific.mpi-cbg.de Fiji - is just ImageJ (Batteries Included)
http://www.chalkie.org.uk Dan's Homepages
https://ifn.mpi-cbg.de Dresden Imaging Facility Network
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )