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January 2020

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Subject:
Re: cmos camera: appearance of boxes/bubbles over images
From:
"Blaauwen, Tanneke den" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 10 Jan 2020 16:13:13 +0000
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Test whether you see it also if you only have an object glass. We had the same with an hamamatsu camera and got a replacement. Condense in the camera. For the rest the camara is very good : >)

Tanneke


On 10 Jan 2020, at 17:05, Oshel, Philip Eugene <[log in to unmask]<mailto:[log in to unmask]>> wrote:

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Do you see these features after some period of observing with the binocs (your eyes) and not using the camera? If so, I'd suspect your mounting medium.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> on behalf of Laurel Ballantyne <[log in to unmask]>
Reply-To: Confocal Microscopy List <[log in to unmask]>
Date: Friday,  10January, 2020 at 10:55
To: "[log in to unmask]" <[log in to unmask]>
Subject: cmos camera: appearance of boxes/bubbles over images

   *****
   To join, leave or search the confocal microscopy listserv, go to:
   http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
   Post images on http://www.imgur.com and include the link in your posting.
   *****

   Images can be seen here,
   https://imgur.com/a/ff7aePj

   Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!



Guardare meravigliarsi et tornar a guardare

Tanneke den Blaauwen
Assoc prof Bacterial Cell Biology

Visiting address:
SILS, UvA
Science Park 904, C3.108
1098 XH Amsterdam, NL
[log in to unmask]<mailto:[log in to unmask]>
Phone 0031-205253852

Mail address:
T. den Blaauwen
Bacterial Cell Biology
SILS
PO BOX 94215
1090 GE Amsterdam
The Netherlands

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