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In all honesty I don't think it will help.  As Goran says
the birefringence will screw you up to some extent anyway
but it seems to me your best bet is to keep the cells in
water (or medium) under the coverslip and use a water
objective with correction collar, setting it to a value
proportionally thicker than the actual coverslip thickness
to account for the higher RI.

                                        Guy




>Hi-
>I have a user growing cells on a track-etched polycarbonate filter.  He
>wants to image pseudopods growing on the underside of the filter as well
>as the cell bodies on the other side, so he takes a z-series through the
>entire cell including the filter.  The problem is that the polycarbonate
>has a ri of 1.58-1.59 so it really screws up the images on the far side of
>the filter.  I thought it might be easiest if he used a mounting medium
>that was close to the polycarbonate ri, but haven't been able to find
>anything yet.  Something miscible with water would be preferred especially
>since I don't know how reactive polycarbonate is.  Any ideas?
>
>
>Thanks-
>Sarah
>
>
>-------------------------------------------------------------------------------
>--------
>Sarah Locknar, Ph.D.
>Director, Neuroscience COBRE Imaging / Physiology Core
>College of Medicine, University of Vermont
>E015 Given Building
>89 Beaumont Ave.
>Burlington, VT 05405
>802-656-0413
>802-656-8704 (fax)
>-------------------------------------------------------------------------------
>--------
>
>


Assoc. Prof. Guy Cox,                 ooOOOOOOoo
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