ROBERT ZUCKER wrote:
>
> Can you see fluorescence with a .5x  lenses on a Nikon microscope. It
> must be very bright or the optics of the Nikon are superb. . The NA of
> the lenses must be below 0.1 Please comment on what type of
> fluorescence can be obtained with these lenses?
> Bob Zucker


Yes, you can see fluorescence with a 0.5x Objective! The Objective has
an n.a. of .02 and a w.d. of 7mm. I have successfully seen G.F.P. with
this lens using a typical broad band excitation filter (450-490nm) and
HBO 100W illumination. Moreover, my colleague Charles Fanghella was
stating that this same lens has been incorporated in our PCM-2000
Confocal Laser Scanning Microscope and renders outstanding images over
the largest area with no problems; Argon Laser with 488nm filter.

Regards,

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland
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