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Dear colleagues,

We are now beginning to do ratiometric calcium imaging (Indo-1) on a 
real-time confocal system (VTeye from VisiTech), connected to an 
inverted Nikon Diaphot 200 microscope (160 mm tube length). Since our 
Nikon Plan-Apo 60x water immersion lens does not transmit UV (we are 
using 350 nm from a Spectra-Physics laser), we are looking for a 
corresponding lens (60x or 40x) to do live imaging using UV excitation. 
Does anyone ut there know of such a UV-transmittable lens? The working 
distance beyond the cover glass (=the bottom of our preparation chamber) 
should preferably be around 300 mikrons.

Thanks in advance for any suggestions!

Best regards,
Peter W

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Peter Wallén
Ph.D., Assoc. Prof.

 

Nobel Institute for Neurophysiology,
Department of Neuroscience,

Karolinska Institute

Retzius väg 8, A3:5
SE-171 77 Stockholm, Sweden

 

Phone: +46-8-524 86913

Fax: +46-8-34 95 44
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