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Hi Krzysztof,
This list might be helpful.
https://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficients.pdf
Best,
Suvrajit
On 4 July 2017 at 01:18, Krzysztof Berniak <[log in to unmask]>
wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
>
>
> I am looking for a fluorescent dye with a known diffusion coefficient,
> which can be excited by laser line 594nm. I need it to calibrate and
> determine a confocal volume in Fluorescence Correlation Spectroscopy (FCS)
> measurement where I use mCherry fluorescent protein (unfortunately, I do
> not have access to 561nm line, which is also good for mCherry).
>
> Fluorescent dyes such as Atto 590, Atto 594 or Atto Rho 13 look nice, but I
> have not found their diffusion coefficients in literature.
>
> Any help or suggestions would be greatly appreciated!
>
>
> Regards,
>
> Krzysztof Berniak
>
> Cell Biophysics
>
> Jagiellonian University, Krakow
>
--
Suvrajit Saha
Postdoctoral Scholar
Prof. Orion Weiner Lab
University of California, San Francisco ( UCSF)
Cardiovascular Research Institute (CVRI)
Smith Cardiovascular Research Building, Room 384, MC 3120
555 Mission Bay Blvd. South
San Francisco, CA 94158
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