CONFOCALMICROSCOPY Archives

June 2001

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Subject:
Re: multiphoton laser
From:
Rolf Borlinghaus <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 11 Jun 2001 09:51:39 +0200
Content-Type:
text/plain
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> David Vaudry
> Laboratory of Cellular and Molecular Regulation
wrote:
> I would like to have your opinion concerning pico vs femto.


"...Thus, widening the laser pulse shape at constant average intensity both in time and in focal volume is beneficial for avioding this kind of damage..."

Hopt, A. and Neher E.
Highly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy
Biophysical Journal Vol 80; April 2001; pgg 2029...2036

Rolf


--------------------------------
Dr. R. Th. Borlinghaus
Manager Support and Service

Leica Microsystems Heidelberg GmbH
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Germany

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