In message "Dr. Mark A. Farmer" writes:
> We have had great success in examining aldehyde fixed material that has
> considerable autofluorescence in the fluorescein and rhodamine range.  We
> do this by using the 647 nm excitation line from our MRC 600 equipped with
> an Ar/Kr laser.  By using secondary conjugated to Cy5 (several manufacturers
> available) we are not bothered at all by autofluorescence.
>
> Unfortunately I know of no way of getting around this problem using an Ar
> only.
>
--Does Cy5 help with tissue fixed with glutaraldehyde, or only with
formaldehyde?
 
Also--Quantum Red (Cy5 coupled to phycoerythrin) might be a way of getting
around the Ar-ion laser limitation, assuming that penetration wasn't a
consideration....
 
Thanks--
 
Martin Wessendorf