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Not sure if this would work, but thinkgeek.com has an LED lightbulb that
you can choose colors for.  It's pretty cheap.
http://www.thinkgeek.com/gadgets/lights/831e/?srp=41#tabs

Monica

On Fri, Feb 10, 2012 at 8:10 AM, Cammer, Michael <[log in to unmask]
> wrote:

> A few years ago (2008?) when looking for a cheap UV source I found a LED
> light advertised at UV but really with bright light in the 460-480 nm
> range.  I taped an old FITC filter on the front of a snapshot camera and
> this combo worked fairly well for imaging GFP except that the camera
> overexposed the blue light.
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Steffen Dietzel
> Sent: Friday, February 10, 2012 4:53 AM
> To: [log in to unmask]
> Subject: Re: Photographing and seeing GFP mice
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I am jumping in on this thread with a similar problem: For us it is not
> photographing but seeing the GFP.
>
> We need to distinguish fluorescent from non-fluorescent littermaids.
> This works well under a fluorescence stereo microscope but we would like
> to do the screening with a handheld lamp (in a different room). I
> imagine a blue LED flashlight and a cheap long-pass filter.
> So far I came across two products, for ~ US$ 200 or ~ € 500,
> respectively (http://www.nightsea.com/gfp.htm and
> http://www.clarechemical.com/lamps.htm , the latter company also offers
> camera filters which might be helpful for Jeremy).
>
> I was hoping for something cheaper. Maybe someone found a 'normal'
> bright blue LED flashlight that works for excitation or yet another
> solution?
>
> Steffen
>
>
> On 09.02.2012 18:44, Julio Vazquez wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > If you have access to a fluorescent scanner for green fluorescence
> (Typhoon, etc ...), you may also be able to use that. These are slow
> though, and you probably will need to immobilize the mice through
> anesthesia, or other means.
> >
> > --
> > Julio Vazquez
> > Fred Hutchinson Cancer Research Center
> > Seattle, WA
> >
> > http://www.fhcrc.org
> > ==
> >
> > On Feb 9, 2012, at 5:27 AM, Jeremy Sanderson wrote:
> >
> >> I have to take photographs of GFP newborn and adult m
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Dear Colleagues,
> >>
> >> I have to take photographs of GFP newborn and adult mice to support a
> microscopy paper.This is to show GFP throughout the coat of the newborns,
> and in the tail tips and feet of the adults.
> >>
> >> Normally mice are photographed with a 60mm f# 2.8 lens on a Nikon D70S
> with sync flash at 1/60th or 1/125th sec with an ISO of 100 or 200 set on
> the camera CCD. That, however, is for normal room lighting – plenty of
> photons.
> >>
> >> We don’t want to go above ISO 800 because of noise, yet need to freeze
> the movement of the mice.
> >>
> >> Has anyone done this, and can advise on exposure, whether to use sync
> flash as well as the UV source.
> >>
> >> I have been advised to anaesthetise the mice and photograph out of the
> cage. We'd prefer not to anaesthetise, but there may be no option.
> >>
> >> Any advice, or if you can direct me to someone who has done this. All
> input gratefully received.
> >>
> >> Thanks,
> >> Jeremy Sanderson
> >> Bio-Imaging, MRC Harwell.
> >>
> >
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
>
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