Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

a good tutorial review  by Bolte & Cordelieres  J. Micr, 2006 is very
helpful understanding co-localization analysis.

Mariette

____________________________________________

Dr. M.P.C. Kemner-van de Corput,
____________________________________________

MGC - Dept. of Cell Biology & Genetics
Erasmus Medical Center
Dr. Molewaterplein 50, 3015 GE Rotterdam
POB 2040, 3000 CA Rotterdam, The Netherlands

Office: H-Ee751; tel: +31 10 704.3949
Lab: H-Ee710; tel lab: +31 10 704.3315
tel secr: +31 10 704.3169
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http://www2.eur.nl/fgg/ch1/cellbiology/
http://www.thesis.kemner.biz/
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Op Vr, 28 maart, 2008 10:54 pm, schreef Glen MacDonald:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Colocalization based upon "yellow" could be accurate, if and only if,
> the intensities are comparable and pixel (voxel) quantities in the
> suspected colocalized volumes are in roughly equal.  .  Otherwise,
> the yellow is masked by the predominate channel.  Something small,
> like lysosomes, would need to be sampled properly. Colocalization
> could be masked by blur unless deconvolved, even if images are
> collected with a confocal.
> On Feb 7, 2007, at 1:05 PM, Marc Thibault wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi all,
>>
>> It seems that in many papers from biologists or chemists, and i'm
>> talking
>> high impact factors journals,  colocalisation of two elements is is
>> often
>> assumed  by simple color superposition (ex: red and green fluoresce
>> yellow
>> when colocalising), while microscopists (many physisists I suppose)
>> seem to
>> need a more complex software-based confirmation.
>> Is it ok, when using high end equipment and corrected objectives
>> (apochromat
>> with high NA for ex.), to assume colocalisation by color
>> superposition,
>> especially when fluorophore are confined to small volume entities,
>> like
>> lysosomes ?
>>
>> Thanks
>>
>> Marc
>