***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Oliver, Have you tried to dissolve the crystals in water? Regards, Anne Sent from Mail<https://go.microsoft.com/fwlink/?LinkId=550986> for Windows 10 ________________________________ From: Confocal Microscopy List <[log in to unmask]> on behalf of Oliver Biehlmaier <[log in to unmask]> Sent: Monday, May 28, 2018 8:38:53 AM To: [log in to unmask] Subject: Dirty 20x dipping objective on 2-photon system ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear fellow microscopists, We have very strange dirt / crystals -image on: https://imgur.com/a/PjOnsSC - on a Zeiss dipping objective which we are using on our 2ph-tomography system. We tried to clean it with Ethanol 70%, Ethanol 100%, Chloroform, Ether/Ethanol 30%/70% with zero improvement of the situation. Thus we guess that the dirt is inside the objective, very much likely salt crystals originating from the buffer solution that the objective is dipping in. Have you ever seen something like this? Is it possible that the sealing of the objective is broken after approx. 3 years of dipping and as a consequence these crystals could build up on the rear side of the front lens? All the best, Oliver Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel Phone: +41 61 207 20 73 | Email: [log in to unmask]<mailto:[log in to unmask]> | www.biozentrum.unibas.ch/imcf<http://www.biozentrum.unibas.ch/imcf<http://www.biozentrum.unibas.ch/imcf<http://www.biozentrum.unibas.ch/imcf>> | www.microscopynetwork.unibas.ch<http://www.microscopynetwork.unibas.ch<http://www.microscopynetwork.unibas.ch<http://www.microscopynetwork.unibas.ch>>
