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May 2006

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Subject:
Antifade for TIRF
From:
Simon Walker <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 8 May 2006 09:59:34 -0400
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sorry, this isn't a confocal question, but has anyone used an antifade 
mounting medium that has proven successful in imaging fixed cells using 
TIRF?  My guess is that the usual suspects (Prolong, Vectashield) have too 
high a refractive index.
Thanks in advance,
Simon

Babraham Institute
Cambridge
UK

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