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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I get the same result in flatworms with DAPI--until GVBD, the DNA is too 
diffuse to get a good signal.
Julian

Khaled Machaca wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Tasha,
>
> Don't know about marsupials, but in xenopus when we stain the oocyte 
> one cannot see the GV because the DNA is too diffuse so you don't get 
> a good signal.  One the chromosomes condense after GVBD they are easy 
> to detect.
>
>
> On Sep 1, 2008, at 10:11 AM, Tasha Czarny wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hello
>>
>> I am trying to stain enzymatically dissociated ovarian follicles to
>> determine the oocytes viability using SYBR14 and propidium iodide. I am
>> working in a marsupial which has only one layer of tightly bound
>> cumuls/granulosa cells and I know from previous experiments that the 
>> stain
>> can penetrate the egg.
>>
>> The surrounding cells stain brightly but I cannot find the GV when I 
>> assess
>> with confocal- either by making a z-stack or manually scrolling.
>>
>> Does anyone have any hints on how to see the GV, it should be large and
>> obvious, but my oocytes appear empty with only staining in the 
>> surrounding
>> granulosa cells.
>>
>> Thanks in advance for any help
>>
>> Tasha Czarny
>> PhD student
>> Marsupial Research Lab
>> The University of Newcastle, Australia
>
> -Khaled
>
> Khaled Machaca, PhD
> Associate Professor of Physiology & Biophysics
> Associate Dean for Basic Science Research
> Weill Cornell Medical College Qatar
> Qatar Foundation - Education City
> Tel: +974-492-8423   Fax:  +974-492-8422
> [log in to unmask]
>


-- 
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC  29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)