Hi, You could just probably use Hoescht, to stain the nuclei
I think Dead/ Live uses the same principles and may even use Hoescht!
good luck
Julie

On Thu, 17 Jul 1997, William A. Monroe wrote:

>         Greetings, our laboratory has a customer who wishes to view
> bacterial biofilms on the end of a bolt in our inverted Leica TCS NT CLSM.
> The customer is looking for a fluorescent stain for the bacteria in order
> to visualize them for counting purposes. Is there a simple fluorescent
> stain for this application, or is it necessary to purchase a commercial
> stain kit? A kit called Live/Dead from a commercial vendor has been
> suggested.
>
> Bill Monroe
> EM Technician II
> Mississippi State University
>