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Hi Cosmin,
Check out the following papers that were published by Molecular probes.
They have some data on self-quenching. If I recall correctly, they show the
fluorescence intensity versus degree of labeling curve for  select Alexa
dyes.

J Histochem Cytochem. 1999 Sep;47(9):1179-88.
Alexa dyes, a series of new fluorescent dyes that yield exceptionally
bright, photostable conjugates.
Panchuk-Voloshina N1, Haugland RP, Bishop-Stewart J, Bhalgat MK, Millard
PJ, Mao F, Leung WY, Haugland RP.


J Histochem Cytochem. 2003 Dec;51(12):1699-712.
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes:
fluorescence of the dyes and their bioconjugates.
Berlier JE1, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford
WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP,
Haugland RP.


Hope this info helps.

Sripad


On Thu, Feb 26, 2015 at 5:47 PM, Cosmin Mihai <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> I was looking for data on self-quenching and fluorophore density, and
> could not find anything published on Alexa dyes.
>
>
> For my application the fluorophores are confined in liposomes(~ 100 nm
> diameter) and would like to figure out up to what number theintensity stays
> in the linear range.
>
>
> I am currently using Alexa 488, if there are other organicdyes more
> resistant to self-quenching would welcome any suggestions.
>
>
>
> Thank you,
>
> Cosmin
>