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March 2008

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Subject:
From:
Hege Avsnes Dale <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 7 Mar 2008 16:22:06 +0100
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Hi.
Do anyone have a protocol for good preservation of GFP in tissue after 
freeze sectioning?
The tissue is a tumor with EGFP-expressing cells and need only a 
fixation that will preserve GFP (no permabilization  needed). Would it 
also be a better idea to fix before sectioning?

Hege

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