Hi all,

Does anyone know if Olympus are still only selling that objective with Olympus MPE systems or have they opened it up to anyone to buy?

(Nikon will sell theirs to anyone :) (but I haven't heard any feedback from users yet)).

Regards,

Adrian

On 23/08/2010, at 9:36 AM, Cameron Nowell wrote:

> Hi Adrian,
> 
> We have one of the new Olympus 25x 1.05NA objective installed. In short
> it is absolutely amazing. The image quality, s/n ratio etc. compared to
> our older 20x 0.95NA is phenomenally better. We have reduced excitation
> power by about 1/2 to 2/3 on our regular samples (zebrafish expressing
> GFP) @920nm. The resolution while technically only slightly improved
> looks a hell of a lot better. The correction collar is also a major
> advantage as we can now compensate for the embedding media of out
> samples some what.
> 
> I can answer some, but not all of your questions.
> 
> - Transmission of the objective is meant to be ~82% out to 1000nm, not
> sure what it is at 1400nm but since out MaiTai doesn't tune that far I
> haven't had to find out.
> - The field number is 27.5. This is achieved by creating a "virtual"
> back plane inside the microscope somewhere. The field is not any larger
> than the 20x though due to the system being limited to 18mm FN (though
> this could be higher in the current generation FV1000 MPE systems that
> have an adaptable excitation path).
> - Not sure about the overfill question, hopefully Olympus can chip in
> with that answer.
> - The thread is identical to the older 20x lens. So if you want a bigger
> filed you can always swap them over (assuming you have the adaptable
> light path, otherwise you will have reduced performance.)
> 
> Cheers
> 
> Cam
> 
> 
> 
> Cameron J. Nowell
> Microscopy Manager 
> Centre for Advanced Microscopy 
> Ludwig Institute for Cancer Research 
> PO Box 2008 
> Royal Melbourne Hospital 
> Victoria, 3050 
> AUSTRALIA 
> Office: +61 3 9341 3155 
> Mobile: +61422882700 
> Fax: +61 3 9341 3104 
> Facility Website
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Adrian Negrean
> Sent: Sunday, 22 August 2010 11:17 PM
> To: [log in to unmask]
> Subject: New microscope objectives
> 
> Hi all,
> 
> Does anybody know what are the specs for the new Olympus 25x 1.05 N.A.
> objective or the Nikon 25x 1.1 N.A. objective ?
> 
> More specifically:
> 
> - What is the transmission of the objective up to 1400 nm ?
> - From what I know all Olympus objectives are designed to be used with a
> 180
> mm tube lens. Is this also for this Olympus objective ? Then the
> effective
> focal length of the objective would be 7.2 mm.
> - What is the Field Number of the objective ? Suppose this is 22 mm like
> for
> the 20x 1.0 NA, is it correct then that the diameter of the image plane
> of
> the sample for which the objective is designed is 22/25 mm=0.88 mm.
> - What is the diameter of the back-aperture that has to be overfilled
> with
> the laser beam to get the best PSF ? I intend to overfill it with a
> truncation ratio of 1.
> - What is the location of the paraxial lens equivalent of the objective
> within the objective ?
> - Is the thread type of this objective the same as for the 20x 1.0 N.A.
> ? 
> 
> Did anybody work with these objectives so far ? What is your experience
> ?
> 
> Thanks!
> -- 
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> Nabble.com.
> 
> 
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