Hi Louis,
 
We are using a mitotic cyclin promoter with gus and a destruction box to visualize the dividing cells. For sure DNA replication is not a marker for certain cell division. Just look en endoreduplication
 
Joost

	-----Original Message----- 
	From: Confocal Microscopy List on behalf of Louis Villeneuve 
	Sent: Wed 12/3/2003 3:43 PM 
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	Subject: Cell division markers
	
	
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	Hi everyone,
	 
	We are looking for a marker for cell division (mitosis).  I have read in litterature that an antibody, BRDU, is used for looking at DNA synthesis and therefore cell division.  Does DNA synthesis implies cell division absolutely?  Could some cell could be positive if a DNA repair mechanism is in action?  
	 
	Is there others antibodies or reagents that can be use?  Fixed cells (tissues) or in living cells?
	 
	Thanks!
	 
	Louis
	 
	Louis R. Villeneuve
	Assistant de recherche - microscope  confocal
	Institut de Cardiologie de Montréal- Centre de Recherche
	5000 est Bélanger, Montréal
	Québec , Canada
	H1T 1C8
	 
	514-376-3330 ext3511
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