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Check connectors...  Unplug and replug all connectors. If that doesn't work then its most likely electronic in the pmt3 power supply or pmt3 preamp stages...

Hope this helps
Mark

On 2/05/2012, at 4:22 PM, Arvydas Matiukas wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> 
> Dear list,
> 
> 
> Ch3 PMT intermitently dies on our LSM510 system. After 2-4 hrs
> of powering on, all image pixels turn to  zero intensity no matter
> what are laser power, pinhole, filter, and gain settings are used.
> Cold reboot does not fix the problem though no error messages
> are reported. The single fix so far is to let the system cool down
> for several hours then it is again working for few more hours.
> Surprisingly all cooling fans are spinning normally as well as  room
> conditioning system.
> 
> 
> 
> 
> We are off service contract so any feedback/advice what could
> be wrong and how to fix it is greatly appreciated.
> 
> 
> Thank you,
> Arvydas
> 
> 
> 
> 
> 
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Imaging Core Facility
> Department of Pharmacology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3159
> Syracuse, NY 13210
> tel.: 315-464-7997
> fax: 315-464-8014
> email: [log in to unmask]