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Dear all,
Thank you very much for your responses. I had no idea that these harp-like
devices exist - those would definitely be helpful. Also, using Cy-gel and
agarose are very helpful ideas.
Thanks again,
Bilal
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Scott, Mark
Sent: Thursday, April 11, 2013 4:23 AM
To: [log in to unmask]
Subject: Re: Biopsy imaging/holding tools
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Dear Bilal,
I've used a few basic solutions in the past, anything from a simple metal
washer to super-gluing the sample to the bottom of a small petri-dish
(assuming the sample isn't precious and recovery required).
Another slightly more technical solution would be to use a product called
Cy-gel (no commercial interest - just what we have here in the facility),
which basically is liquid in the fridge and sets like agarose when it warms
to room-temperature. Failing getting hold of that you could use some low
melting point agarose instead but it will depend on how sensitive your
sample is to heat for that to be applicable.
Hope that helps,
Mark Scott
FILM - Facility for Imaging by Light Microscopy Senior Research Technician
Sir Alexander Fleming Building, desk 408 Imperial College London / South
Kensington Exhibition Road London SW7 2AZ UK
Tel: ++44(0)20-759-49793
E-mail: [log in to unmask]
Website: http://imperial.ac.uk/imagingfacility
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Bilal Malik
Sent: 10 April 2013 17:38
To: [log in to unmask]
Subject: Biopsy imaging/holding tools
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Dear all,
In the process of imaging small (2-4 mm) tissue biopsies under a confocal
microscope with a water-immersion objective, we have had problems holding
the tissue sample down to avoid flotation in water. We have tried using
tweezers with a clip to hold them but are afraid that may damage the biopsy
in some instances, and is not always possible for irregular shaped samples.
Can anyone provide suggestions as to how this problem can be solved either
with a commercial or a custom method?
Thanks so much,
Bilal Malik
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