Hi Lily,

Yes, they will bind each other at the membrane. Adding L221K, F223R 
mutations to the Cerulean and all three mutations to venusYFP may reduce 
this hetero-dimerization (Cerulean has the A206K mutation, as published).

Depending on the protein and the level of overexpression (as many young 
folks are in a "blind love" with a very strong CMV promoter and/or COS-7 or 
293T cells which are transgenic for T-large antigen), CeFP-venusYFP 
co-aggregation is a well known phenomena.

If you have any specific questions, please contact me off-line.

Vitaly

NCI-Frederick,
301-846-6575

----- Original Message ----- 
From: "Koo, Lily (NIH/NIAID) [F]" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, October 10, 2008 9:15 AM
Subject: Cerulean Venus binding possible?


> Hello all,
>
> One of my colleagues is conducting an experiment in which she studies
> the diffusion of her membrane protein tagged with Cerulean; also present
> in her cell is another membrane protein tagged with Venus.  Her control
> experiments seem to suggest a possibility that Cerulean and Venus may
> bind to each other.
>
> Has anyone had similar observations or suspicion, at least in cell
> membranes?
>
> Thanks,
>
> Lily
>