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Hi Hao,

Volocity does have the feature to measure total intensity of the Region of
Interest (ROI). In the measurement tab, you may draw multiple ROIs. All
measurements such as intensity and area will be displayed in the
measurements table below the images.

Hope this helps.


Yi

On Thu, Jul 12, 2012 at 12:43 PM, Wu, Hao <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
> I am wondering how to measure total fluorescence intensity using volocity.
> What I want to do is to circle out different region of a tissue or
> different area of a cell, and measure the total fluorescent intensity and
> compare these numbers. However, when I look at velocity tutorial, i could
> not find such a function.
> Can someone please give some advice?
> thank you so much
> Sincerely
> hao